Tuber aestivum/uncinatum is a truffle largely widespread in much of Europe. To date, there are many dilemmas about its cultivation, especially in relation to two morphological entities T. aestivum Vitt. "sensu stricto" and in T. uncinatum Chatin "sensu stricto", also characterized by different ecological and organoleptic features (differences in sporal morphology, taste and smell). The study of its biology could provide useful information to develop, in perspective, tools to define new mycorrhization techniques, and experimental fruiting and cultivation methods. For these reasons the aim of the present study was to search genes important in truffle life cycle and probably involved in T. aestivum mycorrhiza development. It has been reported that PAT (Perilipin/ADRP/TIP47) family proteins regulate the production of lipid droplets, at the bases of the typical appressorial turgor pressure. Analogously, a similar mechanism could be occurring also in Tuber, and for these reasons we focused our attention on a perilipin-like protein in T. aestivum. Starting from the sequence of Metarhizium anisopliae gene, T. melanosporum and T. aestivum perilipin-like genes were characterized. More interestingly, PCR analyses on T. melanosporum and T. aestivum fruitbody cDNAs revealed for the first time a perilipin-like gene expression in ectomycorrhizal fungi. The role of this gene/protein could be studied through gene expression analyses on in vitro cultivations performed in nutritional starvation, in order to understand the activation mechanisms of lipid metabolism during mycelial hyphal growth and mycorrhization. The knowledge on molecular mechanisms that lead to the formation of ectomycorrhiza represents an interesting challenge necessary to improve the mycorrhization technologies, as well as the carpophores productions.

PAT proteins in the lipid storage regulation in Tuber: T. melanosporum and T. aestivum/uncinatum comparison

AMICUCCI, ANTONELLA;CALCABRINI, CINZIA;SPARVOLI, VALENTINA;POTENZA, LUCIA ANNA MARIA;STOCCHI, VILBERTO;
2013

Abstract

Tuber aestivum/uncinatum is a truffle largely widespread in much of Europe. To date, there are many dilemmas about its cultivation, especially in relation to two morphological entities T. aestivum Vitt. "sensu stricto" and in T. uncinatum Chatin "sensu stricto", also characterized by different ecological and organoleptic features (differences in sporal morphology, taste and smell). The study of its biology could provide useful information to develop, in perspective, tools to define new mycorrhization techniques, and experimental fruiting and cultivation methods. For these reasons the aim of the present study was to search genes important in truffle life cycle and probably involved in T. aestivum mycorrhiza development. It has been reported that PAT (Perilipin/ADRP/TIP47) family proteins regulate the production of lipid droplets, at the bases of the typical appressorial turgor pressure. Analogously, a similar mechanism could be occurring also in Tuber, and for these reasons we focused our attention on a perilipin-like protein in T. aestivum. Starting from the sequence of Metarhizium anisopliae gene, T. melanosporum and T. aestivum perilipin-like genes were characterized. More interestingly, PCR analyses on T. melanosporum and T. aestivum fruitbody cDNAs revealed for the first time a perilipin-like gene expression in ectomycorrhizal fungi. The role of this gene/protein could be studied through gene expression analyses on in vitro cultivations performed in nutritional starvation, in order to understand the activation mechanisms of lipid metabolism during mycelial hyphal growth and mycorrhization. The knowledge on molecular mechanisms that lead to the formation of ectomycorrhiza represents an interesting challenge necessary to improve the mycorrhization technologies, as well as the carpophores productions.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11576/2611398
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