The manipulation of biosynthetic routes is a plant research sector in continuous expansion to meet the worldwide growing demand of health promoting compounds (1). To set up a means for a standardized production of anthocyanins, here we aimed at developing a genetic transformation system to introduce in apple pulp calli the maize helix-loop-helix (bHLH) transcription factor Sn and a marker for kanamycin resistance. Apple pulp calli are in fact competent to acquire a red colour only under continuous light treatment (2). Conversely, when ectopically expressed, Sn gene can boost the accumulation of anthocyanins in different species and under different enviromental conditions (3). To optimize the culture/selection system, apple pulp calli were grown on different kanamycin concentrations and 300mg/L was selected as the optimal one. Meantime, in order to gain a glimpse on the protein profile in this material, we carried out a SDS-PAGE analysis of the total proteins extracted from white (in the dark) and red (in the light) apple pulp calli. Several red calli - specific proteins have been identified and they will be sequenced in the next future. Apple pulp calli transformation with Sn via Agrobacterium tumefaciens is ongoing
Genetic transformation of apple pulp calli as a possible strategy for the production of bioactive compounds
Daniele Fraternale;Andrea Pompa
2019
Abstract
The manipulation of biosynthetic routes is a plant research sector in continuous expansion to meet the worldwide growing demand of health promoting compounds (1). To set up a means for a standardized production of anthocyanins, here we aimed at developing a genetic transformation system to introduce in apple pulp calli the maize helix-loop-helix (bHLH) transcription factor Sn and a marker for kanamycin resistance. Apple pulp calli are in fact competent to acquire a red colour only under continuous light treatment (2). Conversely, when ectopically expressed, Sn gene can boost the accumulation of anthocyanins in different species and under different enviromental conditions (3). To optimize the culture/selection system, apple pulp calli were grown on different kanamycin concentrations and 300mg/L was selected as the optimal one. Meantime, in order to gain a glimpse on the protein profile in this material, we carried out a SDS-PAGE analysis of the total proteins extracted from white (in the dark) and red (in the light) apple pulp calli. Several red calli - specific proteins have been identified and they will be sequenced in the next future. Apple pulp calli transformation with Sn via Agrobacterium tumefaciens is ongoingI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
