Methanolic extracts of micropropagated Rubus ulmifolius plantlets were tested for antimicrobial activity against different strains of food-borne pathogenic bacteria. Crude methanolic extract effectively reduced the growth (up to 70 and 90%) of seven Escherichia coli and five Salmonella strains, and caused a 40% and 10% growth inhibition on one strain of Pseudomonas spp. and Listeria monocytogenes, respectively. Tannin-rich fractions, obtained by crude extract fractionation, were active against E. coli (about 65% inhibition), while fractions containing chlorogenic acid or caftaric acid and caffeoyl derivatives were almost ineffective (about 10% inhibition). The results suggest that in vitro cultures of R. ulmifolius could be a source of secondary metabolites to be used as preservatives in food products and as antimicrobial agents.
Antibacterial activity of a stable standardized in vitro culture of Rubus ulmifolius Schott against food-borne pathogenic bacteria
BRANDI, GIORGIO;AMAGLIANI, GIULIA;SISTI, MAURIZIO;FRATERNALE, DANIELE;NINFALI, PAOLINO;SCOCCIANTI, VALERIA
2007
Abstract
Methanolic extracts of micropropagated Rubus ulmifolius plantlets were tested for antimicrobial activity against different strains of food-borne pathogenic bacteria. Crude methanolic extract effectively reduced the growth (up to 70 and 90%) of seven Escherichia coli and five Salmonella strains, and caused a 40% and 10% growth inhibition on one strain of Pseudomonas spp. and Listeria monocytogenes, respectively. Tannin-rich fractions, obtained by crude extract fractionation, were active against E. coli (about 65% inhibition), while fractions containing chlorogenic acid or caftaric acid and caffeoyl derivatives were almost ineffective (about 10% inhibition). The results suggest that in vitro cultures of R. ulmifolius could be a source of secondary metabolites to be used as preservatives in food products and as antimicrobial agents.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
