Escherichia coli cultures exposed to a 300mT static magnetic field (SMF) were studied in order to analyse possible induced changes in cellular growth and gene expression. Biomasswas evaluated by visible-light spectrometry and gene expression analyses were carried out by use ofRNAarbitrarily primed PCR. The bacterial strain XL-1Blue, cultivated in traditional and modified Luria–Bertani medium, was exposed to SMF generated by permanent neodymium magnetic disks. The results show alterations induced by SMF in terms of increased cell proliferation and changes in gene expression compared with control groups. Three cDNAs were found to be expressed only in the exposed cells, whereas one cDNA was more expressed in the controls. One clone, expressed only in the exposed cells, corresponds to a putative transposase. This is of particular interest in that it suggests that exposure to a magnetic field may stimulate transposition activity
Effects of a static magnetic field on Escherichi coli growth and gene expression.
DE BELLIS, ROBERTA;CUCCHIARINI, LUIGI;
2004
Abstract
Escherichia coli cultures exposed to a 300mT static magnetic field (SMF) were studied in order to analyse possible induced changes in cellular growth and gene expression. Biomasswas evaluated by visible-light spectrometry and gene expression analyses were carried out by use ofRNAarbitrarily primed PCR. The bacterial strain XL-1Blue, cultivated in traditional and modified Luria–Bertani medium, was exposed to SMF generated by permanent neodymium magnetic disks. The results show alterations induced by SMF in terms of increased cell proliferation and changes in gene expression compared with control groups. Three cDNAs were found to be expressed only in the exposed cells, whereas one cDNA was more expressed in the controls. One clone, expressed only in the exposed cells, corresponds to a putative transposase. This is of particular interest in that it suggests that exposure to a magnetic field may stimulate transposition activityI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
