A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy

Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS

CASABIANCA, ANNA;ORLANDI, CHIARA;FRATERNALE, ALESSANDRA;MAGNANI, MAURO
2004

Abstract

A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11576/2504331
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