A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy
Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS
CASABIANCA, ANNA;ORLANDI, CHIARA;FRATERNALE, ALESSANDRA;MAGNANI, MAURO
2004
Abstract
A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copyFile in questo prodotto:
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