This study was performed to establish whether only 2 sessions per week of combined aerobic and resistance exercise are enough to reduce glycated hemoglobin (HbA(1c) and to induce changes in skeletal muscle gene expression in Type 2 diabetes mellitus (DM2) subjects with metabolic syndrome. Eight DM2 subjects underwent a 1-yr exercise program consisting of 2 weekly sessions of 140 min that combined aerobic [at 55-70% of maximal oxygen uptake (VO(2max))] and resistance circuit training [at 60-80% of 1 repetition maximum (RM)]. The training significantly improved VO(2max) (from 33.5 +/- 3.8 ml/kg/min to 38.2 +/- 3.5 ml/kg/min, p=0.0085) and muscle strength (p<0.05). Changes over baseline were significant for HbA(1c), reduced by 0.45% (p=0.0084), fasting blood glucose (from 8.8 +/- 1.5 to 6.9 +/- 2.2 mmol/l, p=0.0132), waist circumference (from 98.9 +/- 4.8 to 95.9 +/- 4.6 cm, p=0.0054), body weight (from 87.5 +/- 10.7 to 85.7 +/- 10.1 kg, p=0.0375), systolic blood pressure (from 137 +/- 15 to 126 +/- 8 mmHg, p=0.0455), total cholesterol (from 220 +/- 24 to 184 +/- 13 mg/dl, p=0.0057), and LDL-cholesterol (from 150 +/- 16 to 105 +/- 15 mg/dl, p=0.0004). Mitochondrial DNA/nuclear DNA ratio at 6 and 12 months did not change. There was a significant increase of mRNA of peroxisome proliferator-activated receptor (PPAR)-gamma after 6 months of training (p=0.024); PPAR alpha mRNA levels were significantly increased at 6 (p=0.035) and 12 months (p=0.044). The mRNA quantification of other genes measured [mitochondrially encoded cytochrome c oxidase subunit II (MTCO2), cytochrome c oxidase subunit Vb (COX5b), PPAR gamma coactivator l alpha (PGC1 alpha), glucose transporter 4 (GLUT 4), forkhead transcription factor BOX O1 (FOXO-1), carnitine palmitoyltransferase 1 (CPT-1), lipoprotein lipase (LPL), and insulin receptor substrate 1 (IRS-1)] did not show significant changes at 6 and 12 months. This study suggests that a twice-per-week frequency of exercise is sufficient to improve glucose control and the expression of skeletal muscle PPAR gamma and PPAR alpha in DM2 subjects with metabolic syndrome.

TWO WEEKLY SESSIONS OF COMBINED AEROBIC AND RESISTANCE EXERCISE ARE SUFFICIENT TO PROVIDE BENEFICIAL EFFECTS IN SUBJECTS WITH TYPE 2 DIABETES MELLITUS AND METABOLIC SYNDROME

GUESCINI, MICHELE;MANTUANO, MICHELA;STOCCHI, VILBERTO;
2010-01-01

Abstract

This study was performed to establish whether only 2 sessions per week of combined aerobic and resistance exercise are enough to reduce glycated hemoglobin (HbA(1c) and to induce changes in skeletal muscle gene expression in Type 2 diabetes mellitus (DM2) subjects with metabolic syndrome. Eight DM2 subjects underwent a 1-yr exercise program consisting of 2 weekly sessions of 140 min that combined aerobic [at 55-70% of maximal oxygen uptake (VO(2max))] and resistance circuit training [at 60-80% of 1 repetition maximum (RM)]. The training significantly improved VO(2max) (from 33.5 +/- 3.8 ml/kg/min to 38.2 +/- 3.5 ml/kg/min, p=0.0085) and muscle strength (p<0.05). Changes over baseline were significant for HbA(1c), reduced by 0.45% (p=0.0084), fasting blood glucose (from 8.8 +/- 1.5 to 6.9 +/- 2.2 mmol/l, p=0.0132), waist circumference (from 98.9 +/- 4.8 to 95.9 +/- 4.6 cm, p=0.0054), body weight (from 87.5 +/- 10.7 to 85.7 +/- 10.1 kg, p=0.0375), systolic blood pressure (from 137 +/- 15 to 126 +/- 8 mmHg, p=0.0455), total cholesterol (from 220 +/- 24 to 184 +/- 13 mg/dl, p=0.0057), and LDL-cholesterol (from 150 +/- 16 to 105 +/- 15 mg/dl, p=0.0004). Mitochondrial DNA/nuclear DNA ratio at 6 and 12 months did not change. There was a significant increase of mRNA of peroxisome proliferator-activated receptor (PPAR)-gamma after 6 months of training (p=0.024); PPAR alpha mRNA levels were significantly increased at 6 (p=0.035) and 12 months (p=0.044). The mRNA quantification of other genes measured [mitochondrially encoded cytochrome c oxidase subunit II (MTCO2), cytochrome c oxidase subunit Vb (COX5b), PPAR gamma coactivator l alpha (PGC1 alpha), glucose transporter 4 (GLUT 4), forkhead transcription factor BOX O1 (FOXO-1), carnitine palmitoyltransferase 1 (CPT-1), lipoprotein lipase (LPL), and insulin receptor substrate 1 (IRS-1)] did not show significant changes at 6 and 12 months. This study suggests that a twice-per-week frequency of exercise is sufficient to improve glucose control and the expression of skeletal muscle PPAR gamma and PPAR alpha in DM2 subjects with metabolic syndrome.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11576/2504907
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