This article describes a nano-scale method for the determination and quantification of five benzo-diazepines (BDZ) in an alcoholic grappa drink (chlordiazepoxide; lorazepam; diazepam; oxazepam;medazepam). BDZ are typically used in drug-facilitated crimes (DFC) for their accessibility and syner-gistic effects with alcohol. Specimens collected on the crime scene must be rapidly analyzed to prove thecrime, though, in most cases, a very small amount is available. Off-line MEPS extraction of diluted grappasamples proved to be an efficient and reliable method for the recovery of the selected compounds. Requir-ing a very small amount of extraction solvents, MEPS is an environment-friendly technique. LC separationwith UV detection was used as the analytical technique because it is simple, robust, relatively economicand easy-to-find in most laboratories. The method was validated in terms of precision, accuracy andrecovery. Limits of detection and quantitation were in the range of 0.5–2 ng/L. Linearity (R2) spannedfrom 0.9994 and 1.0000. Intra- and inter-day repeatabilities were lower than 12% at any concentration.Recovery percentages of an equivalent-to-real sample at three different concentrations were between70.7 and 74.1%.
Microextraction by packed sorbent (MEPS)-UHPLC-UV: A simple and efficient method for the determination of five benzodiazepines in an alcoholic beverage
MAGRINI, LAURA;CAPPIELLO, ACHILLE;FAMIGLINI, GIORGIO;PALMA, PIERANGELA
2016
Abstract
This article describes a nano-scale method for the determination and quantification of five benzo-diazepines (BDZ) in an alcoholic grappa drink (chlordiazepoxide; lorazepam; diazepam; oxazepam;medazepam). BDZ are typically used in drug-facilitated crimes (DFC) for their accessibility and syner-gistic effects with alcohol. Specimens collected on the crime scene must be rapidly analyzed to prove thecrime, though, in most cases, a very small amount is available. Off-line MEPS extraction of diluted grappasamples proved to be an efficient and reliable method for the recovery of the selected compounds. Requir-ing a very small amount of extraction solvents, MEPS is an environment-friendly technique. LC separationwith UV detection was used as the analytical technique because it is simple, robust, relatively economicand easy-to-find in most laboratories. The method was validated in terms of precision, accuracy andrecovery. Limits of detection and quantitation were in the range of 0.5–2 ng/L. Linearity (R2) spannedfrom 0.9994 and 1.0000. Intra- and inter-day repeatabilities were lower than 12% at any concentration.Recovery percentages of an equivalent-to-real sample at three different concentrations were between70.7 and 74.1%.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
