Flow cytometry assays for viability and cell death. I. Necrosis, apoptosis and cell proliferation. Today, several assays and antibodies are available for the analysis of apoptosis, cell cycle and DNA repair, cell viability and vitality, proliferation, migration, adhesion, endo- and exocytosis. Many of the assays are fluorescence- or colorimetricbased, offering sensitivity, practicability, as well as safety. These reagents have been validated on multiple instrument platforms, including microscopy and flow cytometry analysers. Here we describe some advances in application of flow cytometry particularly related to cell cycle analysis and cell proliferation studies, giving some general information and reporting protocols currently used in laboratories. Particularly, fluorescent probes have been organized into categories based on different aspects of apoptosis, necrosis and cell proliferation.
le colture cellulari in ambito biomedico-diagnostico
LUCHETTI, FRANCESCA;CANONICO, BARBARA;PAPA, STEFANO
2009
Abstract
Flow cytometry assays for viability and cell death. I. Necrosis, apoptosis and cell proliferation. Today, several assays and antibodies are available for the analysis of apoptosis, cell cycle and DNA repair, cell viability and vitality, proliferation, migration, adhesion, endo- and exocytosis. Many of the assays are fluorescence- or colorimetricbased, offering sensitivity, practicability, as well as safety. These reagents have been validated on multiple instrument platforms, including microscopy and flow cytometry analysers. Here we describe some advances in application of flow cytometry particularly related to cell cycle analysis and cell proliferation studies, giving some general information and reporting protocols currently used in laboratories. Particularly, fluorescent probes have been organized into categories based on different aspects of apoptosis, necrosis and cell proliferation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
