A novel liquid chromatography-mass spectrometry (LC-MS) interfacing concept is presented and discussed. The new interface, called Liquid-EI (LEI), is based on electron ionization (EI) but, differently from any previous attempt, the vaporization of solutes and mobile phase takes place at atmospheric pressure into a specifically designed region, called “vaporization micro-channel”, before entering the high-vacuum ion source. The interface is completely independent from the rest of the instrumentation, and can be adapted to any gas chromatography-mass spectrometry (GC-MS) system, as an add-on for a rapid LC-MS conversion. Pressure drop and temperature gradient between LC and MS were considered to enhance the analyte response and reduce band broadening and/or solute carryovers. A fused silica liner, placed inside the vaporization micro-channel, acts as an inert vaporization surface speeding up the gas-phase conversion of large molecules while lessening possible memory effects. The liner is easily replaceable for a quick and extremely simple interface maintenance. Proof of concept and detailed description of the interface are here presented.

Atmospheric Pressure Vaporization Mechanism for Coupling a Liquid Phase with Electron Ionization Mass Spectrometry

TERMOPOLI, VERONICA;FAMIGLINI, GIORGIO;PALMA, PIERANGELA;PIERGIOVANNI, MAURIZIO;CAPPIELLO, ACHILLE
2017-01-01

Abstract

A novel liquid chromatography-mass spectrometry (LC-MS) interfacing concept is presented and discussed. The new interface, called Liquid-EI (LEI), is based on electron ionization (EI) but, differently from any previous attempt, the vaporization of solutes and mobile phase takes place at atmospheric pressure into a specifically designed region, called “vaporization micro-channel”, before entering the high-vacuum ion source. The interface is completely independent from the rest of the instrumentation, and can be adapted to any gas chromatography-mass spectrometry (GC-MS) system, as an add-on for a rapid LC-MS conversion. Pressure drop and temperature gradient between LC and MS were considered to enhance the analyte response and reduce band broadening and/or solute carryovers. A fused silica liner, placed inside the vaporization micro-channel, acts as an inert vaporization surface speeding up the gas-phase conversion of large molecules while lessening possible memory effects. The liner is easily replaceable for a quick and extremely simple interface maintenance. Proof of concept and detailed description of the interface are here presented.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11576/2641829
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