The well-documented relationship between sperm oxidation and male infertility strongly encourages the development of assays for reactive oxygen species detection in semen samples. The present study aims to apply the microplate-based 2',7'-dichlorofluorescein diacetate assay to the evaluation of oxidative stress in unprocessed whole semen, thus avoiding sample centrifugations and other manipulations that may cause significant reactive oxygen species increments.

A microplate-based DCFH-DA assay for the evaluation of oxidative stress in whole semen

Benedetti, Serena
;
Catalani, Simona;Fraternale, Alessandra;Palma, Francesco;
2022

Abstract

The well-documented relationship between sperm oxidation and male infertility strongly encourages the development of assays for reactive oxygen species detection in semen samples. The present study aims to apply the microplate-based 2',7'-dichlorofluorescein diacetate assay to the evaluation of oxidative stress in unprocessed whole semen, thus avoiding sample centrifugations and other manipulations that may cause significant reactive oxygen species increments.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11576/2705091
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