SISMES, XIII Congresso Nazionale SISMES, 4-6 November 2022, Milano

Purpose: The use of cell culture models in exercise oncology has recently received growing interest [1], offering the opportunity to evaluate the effects of different exercise types and intensities and to identify physiological predictors of those effects. We have previously shown that conditioned serum, obtained after high-intensity endur- ance cycling sessions performed by healthy young women, markedly impacted the microtumor-forming capacity (3D growth) of cancer cells [2]. Here, the effects of a home-based lifestyle intervention program and supervised exercise in breast cancer survivors (BCS) are presented. Methods: Conditioned sera were obtained from sedentary BCS enrolled in two ongoing clinical trials. The first trial (MoviS, NCT04818359) aimed to evaluate the effects of lifestyle interventions on the quality of life of BCS who, in the first recruitment round (30 subjects), followed a 12-week home-based, remotely supervised, moderate-intensity aerobic exercise training program. The second trial (20 subjects) aimed to evaluate the effects of two on-site supervised aerobic exercise sessions performed at moderate and vigorous intensity. Serum samples were used to induce 3D microtumor growth in a semi-solid medium. Results: Results from the first trial revealed that 16 out of 30 serum samples obtained after the intervention training showed a [ 5% of reduction in microtumor formation capacity concerning serum collected before (21.2% on average). Considering samples from all 30 BCS, the average reduction was 10.2% (p [ 0.01). Interestingly, statistical analyses revealed that IGF-1 was the only predictor of microtumor formation. Preliminary results from the second trial revealed that the microtumor formation induced by serum collected after the exercise sessions is 10-15% less than that induced by serum taken at rest, without any significant differences between exercise intensity. Conclusions: These results demonstrate the potential of a lifestyle intervention program in tumor progression control. The precise evaluation of the effects induced by exercise and lifestyle changes about physio/metabolic parameters could allow us to identify predictors useful for cancer prevention. Improving our understanding of the relationship between physical exercise and cancer progression will be useful in optimizing recommendations and exercise protocols for cancer recurrence prevention. References 1. Metcalfe RS, Kemp R et al. Anti-carcinogenic effects of exercise- conditioned human serum: evidence, relevance, and opportunities. Eur J Appl Physiol 2021. 121, 2107–2124. 2. Baldelli G, De Santi M, et al. The effects of human sera conditioned by high intensity exercise sessions and training on the tumorigenic potential of cancer cells. Clin Transl Oncol 2021. 23(1):22-34.